Evaluation of Necessity of Cholesterol Supplementation in Diets of Two Marine Teleosts, Turbot (Scophthalmus maximus) and Tiger Puffer (Takifugu rubripes): Effects on Growth and Lipid Metabolism

Two 70-day feeding trials were conducted to reevaluate the necessity of cholesterol supplementation in diets two important marine fish species, turbot and tiger puffer. Graded levels (0, 0.5, 1.0, 2.0, 4.0% dry matter) added into basal diet (with 30% fishmeal but no oil) make five experimental diets, which designated as control, CHO-0.5, CHO-1.0, CHO-2.0, CHO-4.0, respectively. The dietary concentration was measured be 0.11%, 0.65%, 1.10%, 2.32%, 4.59%, Each group had triplicate tanks, each tank stocked with 30 fish. results showed that compared significant effect on growth both Nevertheless, excess (CHO-2.0 CHO-4.0) led lower weight gain puffer CHO-1.0. feed intake decreased increasing cholesterol. In general, crude lipid content liver, whereas 0.5% tended increase muscle species. Dietary significantly regulated lipid-related biochemical parameters serum, muscle, turbot, compositions a higher buffering capacity response changes level. increased 20:4n-6 liver. downregulated gene expression HMG-COAr, upregulated CYP7A1 expression, absorption biosynthesis genes, CPT1 transport-related genes (ApoA1, ApoA4, ApoB100, ApoEα) conclusion, under present condition, accumulation metabolism.